Some synonymous and nonsynonymous gyrA mutations in Mycobacterium tuberculosis lead to systematic false-positive fluoroquinolone resistance results with the Hain GenoType MTBDRsl assays

Some synonymous and nonsynonymous gyrA mutations in Mycobacterium tuberculosis lead to systematic false-positive fluoroquinolone resistance results with the Hain GenoType MTBDRsl assays

In this study, using the Hain GenoType MTBDRsl assays (versions 1 and 2), we found that some nonsynonymous and synonymous mutations in gyrA in Mycobacterium tuberculosis result in systematic false-resistance results to fluoroquinolones by preventing the binding of wild-type probes. Moreover, such mu...

ver descrição completa

Detalhes bibliográficos
Principais autores: Ajileye, A, Alvarez, N, Merker, M, Walker, T, Akter, S, Brown, K, Moradigaravand, D, Schön, T, Andres, S, Schleusener, V, Omar, S, Coll, F, Huang, H, Diel, R, Ismail, N, Parkhill, J, de Jong, B, Peto, T, Crook, D, Niemann, S, Robledo, J, Smith, E, Peacock, S, Köser, C
Formato: Journal article
Idioma:English
Publicado em: American Society for Microbiology 2017
Descrição
Resumo:In this study, using the Hain GenoType MTBDRsl assays (versions 1 and 2), we found that some nonsynonymous and synonymous mutations in gyrA in Mycobacterium tuberculosis result in systematic false-resistance results to fluoroquinolones by preventing the binding of wild-type probes. Moreover, such mutations can prevent the binding of mutant probes designed for the identification of specific resistance mutations. Although these mutations are likely rare globally, they occur in approximately 7% of multidrug-resistant tuberculosis strains in some settings.

Registros relacionados