$Crystallization and preliminary X-ray diffraction studies on recombinant isopenicillin N synthase from Aspergillus nidulans.$

Crystallization and preliminary X-ray diffraction studies on recombinant isopenicillin N synthase from Aspergillus nidulans.

Recombinant Aspergillus nidulans isopenicillin N synthase was purified from an Escherichia coli expression system. The apoenzyme in the presence of saturating concentrations of MnCl2 could be crystallized by either macro- or microseeding, using the hanging drop vapor diffusion technique with polyeth...

書誌詳細
主要な著者:	Roach, P, Schofield, C, Baldwin, J, Clifton, I, Hajdu, J
フォーマット:	Journal article
言語:	English
出版事項:	1995

類似資料

Glutamine-330 is not essential for activity in isopenicillin N synthase from Aspergillus nidulans.
著者:: Sami, M, 等
出版事項: (1997)

Crystallization and preliminary X-ray diffraction studies on a recombinant isopenicillin N synthase from Cephalosporium acremonium.
著者:: Fujishima, Y, 等
出版事項: (1994)

Structure of isopenicillin N synthase complexed with substrate and the mechanism of penicillin formation.
著者:: Roach, P, 等
出版事項: (1997)

Crystal structure of isopenicillin N synthase is the first from a new structural family of enzymes.
著者:: Roach, P, 等
出版事項: (1995)

Anaerobic crystallisation of an isopenicillin N synthase.Fe(II).substrate complex demonstrated by X-ray studies.
著者:: Roach, P, 等
出版事項: (1996)

EXAFS STUDIES OF ISOPENICILLIN-N SYNTHASE
著者:: Randall, C, 等
出版事項: (1992)

Anaerobic crystallisation of an isopenicillin N synthase center dot Fe(II)center dot substrate complex demonstrated by X-ray studies
著者:: Roach, P, 等
出版事項: (1996)

The crystal structure of isopenicillin N synthase with a dipeptide substrate analogue.
著者:: Daruzzaman, A, 等
出版事項: (2013)

ISOPENICILLIN-N SYNTHASE - A NEW MODE OF REACTIVITY
著者:: Baldwin, J, 等
出版事項: (1991)

ISOPENICILLIN-N SYNTHASE - A NEW MODE OF REACTIVITY
著者:: Baldwin, J, 等
出版事項: (1992)

The crystal structure of an LLL-configured depsipeptide substrate analogue bound to isopenicillin N synthase.
著者:: Ge, W, 等
出版事項: (2010)

Structural studies on the reaction of isopenicillin N synthase with the substrate analogue delta-(l-alpha-aminoadipoyl)-l-cysteinyl-d-alpha-aminobutyrate.
著者:: Long, A, 等
出版事項: (2003)

FORMATION OF A NOVEL BICYCLIC GAMMA-LACTAM WITH ISOPENICILLIN-N SYNTHASE
著者:: Baldwin, J, 等
出版事項: (1989)

Identification of many crystal forms of Aspergillus nidulans dehydroquinate synthase.
著者:: Nichols, C, 等
出版事項: (2001)

GAMMA-LACTAM FORMATION FROM TRIPEPTIDES WITH ISOPENICILLIN-N SYNTHASE
著者:: Baldwin, J, 等
出版事項: (1988)

Analysis of the conversion of delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-alpha-aminobutyrate by active-site mutants of Aspergillus nidulans isopenicillin N synthase.
著者:: Rowe, C, 等
出版事項: (1998)

Molecular weight analysis of isopenicillin N synthase by electrospray mass spectrometry.
著者:: Aplin, RT, 等
出版事項: (1990)

X-ray absorption studies of the ferrous active site of isopenicillin N synthase and related model complexes.
著者:: Randall, C, 等
出版事項: (1993)

Studies on side chain interactions during the isopenicillin N synthase catalysed biosynthesis of penicillins
著者:: Cavelier, F, 等
出版事項: (1997)

Purification, crystallization and preliminary X-ray diffraction of anthocyanidin synthase from Arabidopsis thaliana.
著者:: Turnbull, J, 等
出版事項: (2001)

Studies of isopenicillin N synthase enzymatic properties using a continuous spectrophotometric assay.
著者:: Dubus, A, 等
出版事項: (2000)

Structural studies on the reaction of isopenicillin N synthase with the truncated substrate analogues delta-(L-alpha-aminoadipoyl)-L-cysteinyl-glycine and delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-alanine.
著者:: Long, A, 等
出版事項: (2005)

The crystal structure of isopenicillin N synthase with δ-((L)-α-aminoadipoyl)-(L)-cysteinyl-(D)-methionine reveals thioether coordination to iron.
著者:: Clifton, I, 等
出版事項: (2011)

Crystallographic studies on the binding of selectively deuterated LLD- and LLL-substrate epimers by isopenicillin N synthase.
著者:: Ge, W, 等
出版事項: (2010)

Mechanistic studies on isopenicillin n synthase
著者:: Abbott, S
出版事項: (1991)

Structural studies on the reaction of isopenicillin N synthase with a sterically demanding depsipeptide substrate analogue.
著者:: Ge, W, 等
出版事項: (2009)

Roles of 2-oxoglutarate oxygenases and isopenicillin N synthase in ß-lactam biosynthesis
著者:: Rabe, P, 等
出版事項: (2018)

Mitotic recombination accelerates adaptation in the fungus Aspergillus nidulans.
著者:: Sijmen E Schoustra, 等
出版事項: (2007-04-01)

X-ray free-electron laser studies reveal correlated motion during isopenicillin N synthase catalysis
著者:: Rabe, P, 等
出版事項: (2021)

Modification of iron binding ligands in isopenicillin n synthase
著者:: Sami, M, 等
出版事項: (1998)

Investigations into the post-translational modification and mechanism of isopenicillin N:acyl-CoA acyltransferase using electrospray mass spectrometry.
著者:: Aplin, RT, 等
出版事項: (1993)

Isopenicillin N synthase binds δ-(L-α-aminoadipoyl)-L-cysteinyl-D-thia-allo-isoleucine through both sulfur atoms.
著者:: Clifton, I, 等
出版事項: (2011)

The interaction of isopenicillin N synthase with homologated substrate analogues δ-(L-α-aminoadipoyl)-L-homocysteinyl-D-Xaa characterised by protein crystallography.
著者:: Daruzzaman, A, 等
出版事項: (2013)

Asexual Recombination in a uvsH Mutant of Aspergillus nidulans
著者:: S A SOUZA-JÚNIOR, 等
出版事項: (2007-01-01)

Isopenicillin N synthase mediates thiolate oxidation to sulfenate in a depsipeptide substrate analogue: implications for oxygen binding and a link to nitrile hydratase?
著者:: Ge, W, 等
出版事項: (2008)

Spectroscopic studies reveal details of substrate-induced conformational changes distant from the active site in isopenicillin N synthase
著者:: Rabe, P, 等
出版事項: (2022)

Photoaffinity labelling of isopenicillin N synthetase.
著者:: Baldwin, J, 等
出版事項: (1990)

Increase in mitotic recombination in diploid cells of Aspergillus nidulans in response to ethidium bromide
著者:: Tânia C.A. Becker, 等
出版事項: (2003-01-01)

Ligand-induced conformational changes and a mechanism for domain closure in Aspergillus nidulans dehydroquinate synthase.
著者:: Nichols, C, 等
出版事項: (2003)

Acyl coenzyme A: 6-aminopenicillanic acid acyltransferase from Penicillium chrysogenum and Aspergillus nidulans.
著者:: Whiteman, P, 等
出版事項: (1990)