Ultrasensitive detection and phenotyping of CD4+ T cells with optimized HLA class II tetramer staining.

HLA class I tetramers have revolutionized the study of Ag-specific CD8+ T cell responses. Technical problems and the rarity of Ag-specific CD4+ Th cells have not allowed the potential of HLA class II tetramers to be fully realized. Here, we optimize HLA class II tetramer staining methods through the...

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Main Authors: Scriba, T, Purbhoo, M, Day, C, Robinson, N, Fidler, S, Fox, J, Weber, J, Klenerman, P, Sewell, A, Phillips, R
格式: Journal article
语言:English
出版: 2005