Response surface methodology as a tool to study the lipase-catalyzed synthesis of betulinic acid ester
BACKGROUND: The synthesis of betulinic acid ester using betulinic acid and oleyl alcohol catalyzed by Novozym 435 (immobilized Candida antarctica lipase) was carried out. Response surface methodology (RSM) based on a five-level, three-variable, central composite rotatable design (CCRD) was employe...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Wiley Blackwell
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/6325/1/Response%20surface%20methodology%20as%20a%20tool%20to%20study%20the%20lipase.pdf |
| Summary: | BACKGROUND: The synthesis of betulinic acid ester using betulinic acid and oleyl alcohol catalyzed by Novozym
435 (immobilized Candida antarctica lipase) was carried out. Response surface methodology (RSM) based on a
five-level, three-variable, central composite rotatable design (CCRD) was employed to evaluate the interactive
effects of various parameters. The parameters were reaction time (8–16 h), temperature (20–60 ◦C) and enzyme
amount (120–160 mg).
RESULTS: Simultaneously increasing reaction time, temperature and amount of enzyme increased the yields of
betulinic acid ester produced.
CONCLUSION: The optimum conditions derived via RSM for the reaction were reaction time of 10.2 h,
temperature of 53.1 ◦C and enzyme amount of 138 mg. The actual experimental yield was 48.5% under optimum
conditions, which compared well with the maximum predicted value of 47.6%. |
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