Red light, green light: probing single molecules using alternating-laser excitation.

Single-molecule fluorescence methods, particularly single-molecule FRET (fluorescence resonance energy transfer), have provided novel insights into the structure, interactions and dynamics of biological systems. ALEX (alternating-laser excitation) spectroscopy is a new method that extends single-mol...

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Autori principali: Santoso, Y, Hwang, L, Le Reste, L, Kapanidis, A
Natura: Journal article
Lingua:English
Pubblicazione: 2008