Tentative exploration of pharmacodynamic substances: Pharmacological effects, chemical compositions, and multi-components pharmacokinetic characteristics of ESZWD in CHF-HKYd rats

The chemical components of Xin'an famous prescription Ershen Zhenwu Decoction (ESZWD) are still unclear. The results showed that ESZWD could significantly reduce left ventricular end diastolic diameter, decrease N-terminal pro-brain natriuretic peptide (NT-proBNP), angiotensinII, aldosterone, r...

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Main Authors: Li-li Hong, Yan Zhao, Wei-dong Chen, Chen-yu Yang, Guo-zhuan Li, Hong-song Wang, Xiao-yu Cheng
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-09-01
Series:Frontiers in Cardiovascular Medicine
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fcvm.2022.913661/full
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author Li-li Hong
Li-li Hong
Li-li Hong
Li-li Hong
Yan Zhao
Yan Zhao
Yan Zhao
Yan Zhao
Wei-dong Chen
Wei-dong Chen
Wei-dong Chen
Wei-dong Chen
Chen-yu Yang
Chen-yu Yang
Chen-yu Yang
Chen-yu Yang
Guo-zhuan Li
Guo-zhuan Li
Guo-zhuan Li
Guo-zhuan Li
Hong-song Wang
Hong-song Wang
Hong-song Wang
Hong-song Wang
Xiao-yu Cheng
author_facet Li-li Hong
Li-li Hong
Li-li Hong
Li-li Hong
Yan Zhao
Yan Zhao
Yan Zhao
Yan Zhao
Wei-dong Chen
Wei-dong Chen
Wei-dong Chen
Wei-dong Chen
Chen-yu Yang
Chen-yu Yang
Chen-yu Yang
Chen-yu Yang
Guo-zhuan Li
Guo-zhuan Li
Guo-zhuan Li
Guo-zhuan Li
Hong-song Wang
Hong-song Wang
Hong-song Wang
Hong-song Wang
Xiao-yu Cheng
author_sort Li-li Hong
collection DOAJ
description The chemical components of Xin'an famous prescription Ershen Zhenwu Decoction (ESZWD) are still unclear. The results showed that ESZWD could significantly reduce left ventricular end diastolic diameter, decrease N-terminal pro-brain natriuretic peptide (NT-proBNP), angiotensinII, aldosterone, reactive oxygen species, and malondialdehyde, increase serum superoxide dismutase, while had no significant effect on inflammatory factors. Ultra-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry (UPLC/Q-TOF-MS) analysis detected 30 prototype components in model rats' serum, mainly including alkaloids, saponins, terpenoids, tanshinones, phenols. UPLC-MS/MS successfully detected the pharmacokinetic parameters of four components, and correlation analysis shows that there are negative correlations between four compounds and serum NT-proBNP. Thirty components of ESZWD may play a therapeutic role in chronic heart failure with heart-kidney Yang deficiency (CHF-HKYd) by improving myocardial injury, reducing oxidative stress levels, and inhibiting activation of the RAAS system in rats. Salsolinol, aconitine, paeoniflorin, and miltrione are equipped with potential characteristics as pharmacodynamic substances for ESZWD in treating CHF-HKYd. Additionally, the constituents of ESZWD in CHF-HKYd rats are different from normal rats, which provided a reference for the selection of subjects for further study.
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spelling doaj.art-5dadb19f0aa948adbd6212f21affc5082022-12-22T03:15:34ZengFrontiers Media S.A.Frontiers in Cardiovascular Medicine2297-055X2022-09-01910.3389/fcvm.2022.913661913661Tentative exploration of pharmacodynamic substances: Pharmacological effects, chemical compositions, and multi-components pharmacokinetic characteristics of ESZWD in CHF-HKYd ratsLi-li Hong0Li-li Hong1Li-li Hong2Li-li Hong3Yan Zhao4Yan Zhao5Yan Zhao6Yan Zhao7Wei-dong Chen8Wei-dong Chen9Wei-dong Chen10Wei-dong Chen11Chen-yu Yang12Chen-yu Yang13Chen-yu Yang14Chen-yu Yang15Guo-zhuan Li16Guo-zhuan Li17Guo-zhuan Li18Guo-zhuan Li19Hong-song Wang20Hong-song Wang21Hong-song Wang22Hong-song Wang23Xiao-yu Cheng24School of Pharmacy, Anhui University of Chinese Medicine, Hefei, ChinaAnhui Province Key Laboratory of Chinese Medicinal Formula, School of Pharmacy, Anhui University of Chinese Medicine, Hefei, ChinaSynergetic Innovation Center of Anhui Authentic Chinese Medicine Quality Improvement, School of Pharmacy, Anhui University of Chinese Medicine, Hefei, ChinaMOE-Anhui Joint Collaborative Innovation Center for Quality Improvement of Anhui Genuine Chinese Medicinal Materials, School of Pharmacy, Anhui University of Chinese Medicine, Hefei, ChinaSchool of Pharmacy, Anhui University of Chinese Medicine, Hefei, ChinaAnhui Province Key Laboratory of Chinese Medicinal Formula, School of Pharmacy, Anhui University of Chinese Medicine, Hefei, ChinaSynergetic Innovation Center of Anhui Authentic Chinese Medicine Quality Improvement, School of Pharmacy, Anhui University of Chinese Medicine, Hefei, ChinaMOE-Anhui Joint Collaborative Innovation Center for Quality Improvement of Anhui Genuine Chinese Medicinal Materials, School of Pharmacy, Anhui University of Chinese Medicine, Hefei, ChinaSchool of Pharmacy, Anhui University of Chinese Medicine, Hefei, ChinaAnhui Province Key Laboratory of Chinese Medicinal Formula, School of Pharmacy, Anhui University of Chinese Medicine, Hefei, ChinaSynergetic Innovation Center of Anhui Authentic Chinese Medicine Quality Improvement, School of Pharmacy, Anhui University of Chinese Medicine, Hefei, ChinaMOE-Anhui Joint Collaborative Innovation Center for Quality Improvement of Anhui Genuine Chinese Medicinal Materials, School of Pharmacy, Anhui University of Chinese Medicine, Hefei, ChinaSchool of Pharmacy, Anhui University of Chinese Medicine, Hefei, ChinaAnhui Province Key Laboratory of Chinese Medicinal Formula, School of Pharmacy, Anhui University of Chinese Medicine, Hefei, ChinaSynergetic Innovation Center of Anhui Authentic Chinese Medicine Quality Improvement, School of Pharmacy, Anhui University of Chinese Medicine, Hefei, ChinaMOE-Anhui Joint Collaborative Innovation Center for Quality Improvement of Anhui Genuine Chinese Medicinal Materials, School of Pharmacy, Anhui University of Chinese Medicine, Hefei, ChinaSchool of Pharmacy, Anhui University of Chinese Medicine, Hefei, ChinaAnhui Province Key Laboratory of Chinese Medicinal Formula, School of Pharmacy, Anhui University of Chinese Medicine, Hefei, ChinaSynergetic Innovation Center of Anhui Authentic Chinese Medicine Quality Improvement, School of Pharmacy, Anhui University of Chinese Medicine, Hefei, ChinaMOE-Anhui Joint Collaborative Innovation Center for Quality Improvement of Anhui Genuine Chinese Medicinal Materials, School of Pharmacy, Anhui University of Chinese Medicine, Hefei, ChinaSchool of Pharmacy, Anhui University of Chinese Medicine, Hefei, ChinaAnhui Province Key Laboratory of Chinese Medicinal Formula, School of Pharmacy, Anhui University of Chinese Medicine, Hefei, ChinaSynergetic Innovation Center of Anhui Authentic Chinese Medicine Quality Improvement, School of Pharmacy, Anhui University of Chinese Medicine, Hefei, ChinaMOE-Anhui Joint Collaborative Innovation Center for Quality Improvement of Anhui Genuine Chinese Medicinal Materials, School of Pharmacy, Anhui University of Chinese Medicine, Hefei, ChinaDepartment of Geriatric Cardiology, First Affiliated Hospital of Anhui University of Chinese Medicine, Anhui University of Traditional Chinese Medicine, Hefei, ChinaThe chemical components of Xin'an famous prescription Ershen Zhenwu Decoction (ESZWD) are still unclear. The results showed that ESZWD could significantly reduce left ventricular end diastolic diameter, decrease N-terminal pro-brain natriuretic peptide (NT-proBNP), angiotensinII, aldosterone, reactive oxygen species, and malondialdehyde, increase serum superoxide dismutase, while had no significant effect on inflammatory factors. Ultra-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry (UPLC/Q-TOF-MS) analysis detected 30 prototype components in model rats' serum, mainly including alkaloids, saponins, terpenoids, tanshinones, phenols. UPLC-MS/MS successfully detected the pharmacokinetic parameters of four components, and correlation analysis shows that there are negative correlations between four compounds and serum NT-proBNP. Thirty components of ESZWD may play a therapeutic role in chronic heart failure with heart-kidney Yang deficiency (CHF-HKYd) by improving myocardial injury, reducing oxidative stress levels, and inhibiting activation of the RAAS system in rats. Salsolinol, aconitine, paeoniflorin, and miltrione are equipped with potential characteristics as pharmacodynamic substances for ESZWD in treating CHF-HKYd. Additionally, the constituents of ESZWD in CHF-HKYd rats are different from normal rats, which provided a reference for the selection of subjects for further study.https://www.frontiersin.org/articles/10.3389/fcvm.2022.913661/fullErshen Zhenwu Decoctionchronic heart failure with heart-kidney Yang deficiencyUPLC/Q-TOF-MSpharmacokineticpharmacodynamic substances
spellingShingle Li-li Hong
Li-li Hong
Li-li Hong
Li-li Hong
Yan Zhao
Yan Zhao
Yan Zhao
Yan Zhao
Wei-dong Chen
Wei-dong Chen
Wei-dong Chen
Wei-dong Chen
Chen-yu Yang
Chen-yu Yang
Chen-yu Yang
Chen-yu Yang
Guo-zhuan Li
Guo-zhuan Li
Guo-zhuan Li
Guo-zhuan Li
Hong-song Wang
Hong-song Wang
Hong-song Wang
Hong-song Wang
Xiao-yu Cheng
Tentative exploration of pharmacodynamic substances: Pharmacological effects, chemical compositions, and multi-components pharmacokinetic characteristics of ESZWD in CHF-HKYd rats
Frontiers in Cardiovascular Medicine
Ershen Zhenwu Decoction
chronic heart failure with heart-kidney Yang deficiency
UPLC/Q-TOF-MS
pharmacokinetic
pharmacodynamic substances
title Tentative exploration of pharmacodynamic substances: Pharmacological effects, chemical compositions, and multi-components pharmacokinetic characteristics of ESZWD in CHF-HKYd rats
title_full Tentative exploration of pharmacodynamic substances: Pharmacological effects, chemical compositions, and multi-components pharmacokinetic characteristics of ESZWD in CHF-HKYd rats
title_fullStr Tentative exploration of pharmacodynamic substances: Pharmacological effects, chemical compositions, and multi-components pharmacokinetic characteristics of ESZWD in CHF-HKYd rats
title_full_unstemmed Tentative exploration of pharmacodynamic substances: Pharmacological effects, chemical compositions, and multi-components pharmacokinetic characteristics of ESZWD in CHF-HKYd rats
title_short Tentative exploration of pharmacodynamic substances: Pharmacological effects, chemical compositions, and multi-components pharmacokinetic characteristics of ESZWD in CHF-HKYd rats
title_sort tentative exploration of pharmacodynamic substances pharmacological effects chemical compositions and multi components pharmacokinetic characteristics of eszwd in chf hkyd rats
topic Ershen Zhenwu Decoction
chronic heart failure with heart-kidney Yang deficiency
UPLC/Q-TOF-MS
pharmacokinetic
pharmacodynamic substances
url https://www.frontiersin.org/articles/10.3389/fcvm.2022.913661/full
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